mntH [2019-06-06 10:30:48]

manganese transporter (proton symport)

locus: BSU04360

pI: 9.89

mw: 45.52 kDa

protein length: 425 aa

gene length: 1278 bp

function: manganese uptake

product: manganese transporter (proton symport))

essential: no

synonyms: ydaR

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 491,147 492,424

The protein

Protein family

• NRAMP family (according to Swiss-Prot)

Structure

• [PDB|4WGV] (from ''Staphylococcus capitis'', 40% identity) [Pubmed|25326704]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]
  description: [Pubmed|10760146]

  regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]: repression, [Pubmed|10760146], in [regulon|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR regulon]

  regulation

  • repressed at high Mn(II) concentrations (MntR) [Pubmed|10760146]

Biological materials

Mutant

• MGNA-C116 (ydaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2114 NBRP B. subtilis, Japan]
• JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
• BKE04360 ([gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCACCTGAATTCTG, downstream forward: _UP4_TAAAAAAACCGGCTTCTAAA
• BKK04360 ([gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCACCTGAATTCTG, downstream forward: _UP4_TAAAAAAACCGGCTTCTAAA

labs

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

• 10760146,12950915,10760146,25326704